Selected peer-reviewed articles


Link to PUBMED

Hynes RO, Coller BS, Porteus M. (2017) Toward Responsible Human Genome Editing. JAMA. 2017 Apr 10. doi: 10.1001/jama.2017.4548. PMID 28395003

Glavey SV, Naba A, Manier S, Clauser K, Tahri S, Park J, Reagan MR, Moschetta M, Mishima Y, Gambella M, Rocci A, Sacco A, O'Dwyer ME, Asara JM, Palumbo A, Roccaro AM, Hynes RO, Ghobrial IM. (2017) Proteomic characterization of human multiple myeloma bone marrow extracellular matrix.Leukemia. 2017 Mar 27. doi: 10.1038/leu.2017.102. PMID 2834415

Ragelle H, Naba A, Larson BL, Zhou F, Prijić M, Whittaker CA, Del Rosario A, Langer R, Hynes, Anderson DG.(2017) Comprehensive proteomic characterization of stem cell-derived extracellular matrices.  Biomaterials. 2017 Mar 7;128:147-159. doi: 10.1016/j.biomaterials.2017.03.008 PMID 28327460

Charo RA, Hynes RO (2017) Evolving policy with science. Science. 2017 Mar 3;355(6328):889. doi: 10.1126/science.aan0509. PMID 28254888

Zhu G, Zhang Q, Reddy EC, Carrim N, Chen Y, Xu XR, Xu M, Wang Y, Hou Y, Ma L, Li Y, Rui M, Petruzziello T, Lavalle C, Stratton TW, Lei X, Reheman A, Chen P, Zhu C, Wilkins JA, Hynes RO, Freedman J, Ni H. (2017) Integrin PSI domain has endogenous thiol isomerase function and is a novel target for anti-platelet therapy. Blood. 2017 Jan 25. pii: blood-2016-07-729400. doi: 10.1182/blood-2016-07-729400. PMID 2812273 

Naba A, Clauser KR, Mani DR, Carr SA, Hynes RO (2017) Quantitative proteomic profiling of the extracellular matrix of pancreatic islets during the angiogenic switch and insulinoma progression. Sci Rep. 2017 Jan 10;7:40495. doi: 10.1038/srep40495.

Li R, Hebert JD, Lee TA, Xing H, Boussommier-Calleja A, Hynes RO, Lauffenburger DA, Kamm RD.(2016) Macrophage-secreted TNFα and TGFβ1 Influence Migration Speed and Persistence of Cancer Cells in 3D Tissue Culture via Independent Pathways. Cancer Res. 2016 Nov 21. pii: canres.0442.2016.

Pucci F, Rickelt S, Newton AP, Garris C, Nunes E, Evavold C, Pfirschke C, Engblom C, Mino-Kenudson M, Hynes RO, Weissleder R, Pittet MJ. (2016) PF4 Promotes Platelet Production and Lung Cancer Growth.Cell Rep. 2016 Nov 8;17(7):1764-1772. doi: 10.1016/j.celrep.2016.10.031.PMID: 27829148

Carmona G, Perera U, Gillett C, Naba A, Law AL, Sharma V.P, Wang J, Wyckoff J, Balsamo M, Mosis F, De Piano M, Monypenny J, Woodman N, McConnell R.E, Mouneimne G, Van Hemelrijck M, Cao Y, Condeelis J, Hynes R.O, Gertler F.B, and Krause M. (2016) Lamellipodin promotes invasive 3D cancer cell migration via regulated interactions with Ena/VASP and SCAR/WAVE. Oncogene. Epub 21 March 2016 doi:10.1038/onc.2016.47.

Chen MB, Lamar JM, Li R, Hynes RO, Kamm RD.(2016) Elucidation of the roles of tumor integrin ß1 in the extravasation stage of the metastasis cascade. Cancer Res. 2016 Mar 17. pii: canres.1325.2015

Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.(2016) αV Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells. Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.

Pfirschke C, Engblom C, Rickelt S, Cortez-Retamozo V, Garris C, Pucci F, Yamazaki T, Poirier-Colame V, Newton A, Redouane Y, Lin YJ, Wojtkiewicz G, Iwamoto Y, Mino-Kenudson M, Huynh TG, Hynes RO, Freeman GJ, Kroemer G, Zitvogel L, Weissleder R, Pittet MJ. (2016) Immunogenic Chemotherapy Sensitizes Tumors to Checkpoint Blockade Therapy. Immunity. 2016 Feb 16;44(2):343-54. doi: 10.1016/j.immuni.2015.11.024. Epub 2016 Feb 9.

Oudin MJ, Jonas O, Kosciuk T, Broye LC, Guido BC, Wyckoff J, Riquelme D, Lamar JM, Asokan SB, Whittaker C, Ma D, Langer R, Cima MJ, Wisinski KB, Hynes RO, Lauffenburger DA, Keely PJ, Bear JE, Gertler FB. (2016) Tumor cell-driven extracellular matrix remodeling enables haptotaxis during metastatic progression.Cancer Discov. 2016 Jan 25. pii: CD-15-1183.

Shaw Bagnall J, Byun S, Begum S, Miyamoto DT, Hecht VC, Maheswaran S, Stott SL, Toner M, Hynes RO, Manalis SR.(2015) Deformability of Tumor Cells versus Blood Cells. Sci Rep. 2015 Dec 18;5:18542. doi: 10.1038/srep18542.

Heilmann S, Ratnakumar K, Langdon EM, Kansler ER, S Kim I, Campbell NR, Perry EB, McMahon AJ, Kaufman CK, van Rooijen E, Lee W, Iacobuzio-Donahue CA, Hynes RO, Zon LI, Xavier JB, White RM.(2015) A Quantitative System for Studying Metastasis Using Transparent Zebrafish. Cancer Res. 2015 Oct 15;75(20):4272-82.

Naba A, Clauser KR, Hynes R.O. (2015) Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis.  J Vis Exp. 2015 Jul 23;(101). doi: 10.3791/53057.

Naba A, Clauser KR, Ding H, Whittaker CA, Carr SA, Hynes RO. (2015) The extracellular matrix: Tools and insights for the "omics" era. Matrix Biol. 2015 Jul 8. pii: S0945-053X(15)00121-3. doi: 10.1016/j.matbio.2015.06.003. 

Murphy PA, Begum S, Hynes R.O. (2015). Tumor angiogenesis in the absence of fibronectins or its cognate integrin receptors. PLoS One. 2015 Mar 25;10(3):e0120872. doi: 10.1371/journal.pone.0120872. eCollection 2015.

Turner CJ, Badu-Nkansah K, Crowley D, van der Flier A, Hynes R.O. (2015). a5 and av integrins cooperate to regulate vascular smooth muscle and neural crest functions in vivo. Development. 2015 Feb 15;142(4):797-808. doi: 10.1242/dev.117572.

Labelle, M., Begum, S. and Hynes, R.O. (2014). Platelets guide the formation of early metastatic niches. Proc. Natl. Acad. Sci. USA. 111(30):E3053-61. doi: 10.1073/pnas.1411082111.

Naba, A., Clauser, K.R., Whittaker, C.A.,Carr, S.A., Tanabe, K.K. and Hynes, R.O. (2014c). Extracellular Matrix Signatures of Human Primary Metastatic Colon Cancers & their Liver Metastases. BMC Cancer. 14:518. doi: 10.1186/1471-2407-14-518.

Murphy, P.A. and Hynes, R.O. (2014). Alternative splicing of endothelial fibronectin is induced by disturbed vascular flow and protects against arterial aneurysms. Arterio., Thromb. and Vasc. Biol. 34(9):2042-50. doi: 10.1161/ATVBAHA.114.303879.

Turner CJ, Badu-Nkansah K, Crowley D, van der Flier A, Hynes RO (2014). Integrin–α5β1 is not required for mural cell functions during development of blood vessels but is required for lymphatic-blood vessel separation and lymphovenous valve formation. Dev. Biol. 392(2):381-92. doi: 10.1016/j.ydbio.2014.05.006.

Naba, A. Clauser, K.R. Lamar, J.M. Carr, S.A. & Hynes, R.O. (2014b). Extracellular Matrix signatures of human mammary carcinoma identify novel metastasis promoters. eLife. 3:e01308. DOI: 10.7554/eLife.01308

Miller PG, Al-Shahrour F, Hartwell KA, Chu LP, Järås M, Puram RV, Puissant A, Callahan KP, Ashton J, McConkey ME, Poveromo LP, Cowley GS, Kharas MG, Labelle M, Shterental S, Fujisaki J, Silberstein L, Alexe G, Al-Hajj MA, Shelton CA, Armstrong SA, Root DE, Scadden DT, Hynes RO, Mukherjee S, Stegmaier K, Jordan CT, Ebert BL. (2013). In Vivo RNAi Screening Identifies a Leukemia-Specific Dependence on Integrin Beta 3 Signaling. Cancer Cell. 24(1):45-58.

Yao X, Labelle M, Lamb CR, Dugan JM, Williamson CA, Spencer DR, Christ KR, Keating RO, Lee WD, Paradis GA, Begum S, Hynes RO, Wittrup KD. (2013). Determination of 35 cell surface antigen levels in malignant pleural effusions identifies CD24 as a marker of disseminated tumor cells. Int J Cancer. 133(12):2925-2933.

Naba, A., Clauser, K.R., Hoersch, S., Liu, H., Carr, S.A. and Hynes, R.O. (2012a). The matrisome: in silico definition and in vivo characterization by proteomics of normal and tumor extracellular matrices. Mol. Cell Proteomics. 11(4):M111.014647. doi: 10.1074/mcp.M111.014647.

Li, L., Welser-Alves, J.V., van der Flier, A., Boroujerdi, A., Hynes, R.O. and Milner, R. (2012). An angiogenic role for the α5β1 integrin in promoting endothelial cell proliferation during cerebral hypoxia. Exp. Neurol. [Epub ahead of print, Jun 18, 2012].

Lamar, JM, Stern, P., Liu, H., Schindler, JW, Jiang, Z. and Hynes, RO. (2012). The Hippo pathway target, YAP, promotes metastasis through its TEAD interaction domain. PNAS 109(37):E2441-50.

Gupton,SL, Riquelme, D, Hughes-Alford, SK, Tadros, J, Rudina, SS, Hynes, RO, Lauffenburger, D. and Gertler, FB. (2012). Mena binds α5 integrin directly and modulates α5β1 function. J. Cell Biol. 198(4):657-676.

Reticker-Flynn, N.E., Braga Malta, D.F., Winslow, M.M., Lamar, J.M., Xu, M.J., Underhill, G.H., Hynes, R.O., Jacks T., Bhatia, S.N. (2012). A combinatorial extracellular matrix platform identifies cell-ECM interactions that correlate with metastasis. Nature Communications, 3:1122-1133.

Liu, H., Ong, S-E., Badu-Nkansah, K., Schindler, J., White, F.M. and Hynes, R.O. (2011). CUB-domain-containing protein 1 (CDCP1) activates Src to promote melanoma metastasis. PNAS 108:1379-1384

Xie, L., Duncan, M. B., Pahler, J., Sugimoto, H., Martino, M., Lively, J., Mundel, T.M., Soubasakos,M., Rubin, K., Takeda, T., Inoue, M., Lawler, J., Hynes, R.O., Hanahan, D. and Kalluri, R. (2011). Counterbalancing Angiogenic Regulatory Factors Control the Rate of Cancer Progression and Survival in a Stage-Specific Manner. Proc. Natl. Acad. Sci. USA, 108(24):9939-9944.

Pulina, M.V., Hou, S.Y., Mittal, A., Julich, A., Whittaker, C.A., Holley, S.A., Hynes, R.O. and Astrof, S. (2011). Essential roles of fibronectin in the development of the left-right embryonic body plan. Dev. Biol. 354(2):208-220.

Yang L, Chen G, Mohanty S, Scott G, Fazal F, Rahman A, Begum S, Hynes RO, Xu L. (2011). GPR56 Regulates VEGF Production and Angiogenesis during Melanoma Progression. Cancer Res. 71(16):5558-5568.

Stenzel, D., Lundkvist, A., Sauvaget, D., Busse, M., Graupera, M., van der Flier, A., Wijelath, E.S., Murray, J., Sobel, M., Costell, M., Takahashi, S., Faessler, R., Yamaguchi, Y., Gutmann, D.H., Hynes, R.O and Gerhardt, H. (2011). Integrin-dependent and independent functions of astrocytic fibronectin in retinal angiogenesis. Development 138(20):4451-4463.

Labelle, M., Begum, S. and Hynes, R.O. (2011). Direct Signaling Between Platelets and Cancer Cells Induces an EMT-Like Transition and Promotes Metastasis. Cancer Cell 20: 576-590.

van der Flier, A., Badu-Nkansah, K., Whittaker, C.A., Crowley, D., Bronson, R.T., Adam Lacy-Hulbert, A. and Hynes, R.O. (2010). Endothelial integrins in developmental angiogenesis and vascular remodeling. Development 137:2439-2449.

Sobolev, O., Stern P., Lacy-Hulbert A., Hynes R. O. (2009). Natural killer cells use selectins for suppression of subcutaneous tumors. Cancer Res. 69:2531-2539.

Shinde, A.V., Bystroff, C., Wang, C., Vincent, P.A., Vogelezang, M.G., Hynes, R.O. and Van De Water, L. (2008). EIIIA (ED-A), the alternatively spliced segment of fibronectin, exhibits cryptic cell adhesive activity to integrin α9β1. J. Biol. Chem. 283: 2858-2870.

Xu, L., Shen, S., Hoshida, Y., Subramanian, A., Ross, K., Brunet, J.P., Ramaswamy, S., Mesirov, J.P. and Hynes, R.O. (2008). Gene expression changes in an animal melanoma model correlate with aggressiveness of human melanoma metastases. Mol Cancer Res. 6:760-769.

McCarty, J.H., Barry, M., Crowley, D., Bronson, R.T., Lacy-Hulbert, A. and Hynes, R.O. (2008). Genetic ablation of αv integrins in epithelial cells of the eyelid skin and conjunctiva leads to squamous cell carcinoma. Am. J. Path. 172:1740-1747.

Stern, P., Astrof, S., Erkeland, S., Schustak, J., Sharp, P.A., and Hynes, R.O. (2008). A system for Cre-regulated RNA interference in vivo. Proc. Natl. Acad. Sci. USA 105: 13895-13900.

Zou, Z., Chen, H., Schmaier, A.A., Hynes R.O. and Kahn, M.L. (2007). In vivo structure-function analysis reveals discrete β3 integrin inside-out and outside-in signaling pathways in platelets. Blood 109: 3284-3290.

Astrof, S., Kirby, A., Lindblad-Toh, K., Daly, M.J. and Hynes, R.O. (2007). Heart development in fibronectin-null mice is governed by a genetic modifier on chromosome four. Mech. Dev. 124: 551-8.

Wong, S.Y., Haack, H., Kissil, J.L., Barry, M., Bronson, R.T., Shen, S.S., Whittaker, C.A., Crowley, D, and Hynes, R.O. (2007). Protein 4.1B suppresses prostate cancer progression and metastasis. Proc. Natl. Acad. Sci. USA 104: 12784-12789.

Astrof, S., Crowley, D. and Hynes, R.O. (2007). Multiple cardiovascular defects caused by the absence of alternatively spliced segments of fibronectin. Devel. Biol. 311: 11-24.

Lacy-Hulbert, A., Smith, A.M., Tissire, H., Barry, M., Crowley, D., Bronson, R.T., Roes, J.T., Savill, J.S. and Hynes, R.O. (2007). Ulcerative colitis and impaired clearance of apoptotic cells induced by loss of myeloid av integrins. Proc. Natl. Acad. Sci. USA 104: 15823-15828.

Taverna, D., Crowley, D., Connolly, M., Bronson, R.T. and Hynes, R.O. (2005). A direct test of potential roles for b3 and b5 integrins in growth and metastasis of murine mammary carcinomas. Cancer Res. 65: 10324-10329.

Ni, H., Chen, P., Spring, C.M., Semple, J.W., Lazarus, A.H., Hynes, R.O. and Freedman, J. (2006). A novel murine model of fetal and neonatal alloimmune thrombocytopenia: response to intravenous IgG therapy. Blood 107:2976-2983.

Matuskova, J., Chauhan, A.K., Cambien, B., Astrof, S., Dole, V.S., Piffath, C.L., Hynes, R.O. and Wagner, D.D. (2006). Decreased plasma fibronectin leads to delayed thrombus growth in injured arterioles. Arteriosclerosis, Thrombosis and Vasc. Biol. 26: 1391-1396.

Xu, L., Begum, S., Hearn, J.D. and Hynes, R. O. (2006). GPR56, an atypical G protein-coupled receptor, binds tissue transglutaminase, TG2, and inhibits melanoma tumor growth and metastasis. Proc. Natl. Acad. Sci. USA. 103: 9023-9028.

Sea Urchin Genome Sequencing Consortium, (2006). The genome of the sea urchin Strongylocentrotus purpuratus. Science 314:941-952.

Whittaker, C.A., Bergeron, K.F., Whittle, J., Brandhorst, B.P., Burke, R.D. and Hynes R. O. (2006). The echinoderm adhesome. Dev. Biol. 300: 252-266.

McCarty, J.H., Lacy-Hulbert, A., Charest, A., Bronson, R.T., Crowley, D., Housman, D.H., Savill, J. and Hynes, R.O. (2005). Selective ablation of αv integrins in the central nervous system leads to cerebral hemorrhage, seizures, axonal degeneration & premature death. Development 132:165-76.

Reynolds, L., Conti, F.A., Lucas, M. Grose, R., Robinson, S., Stone, M., Saunders, G.,C. Dickson, C., Hynes, R.O., Lacy-Hulbert, A. and Hodivala-Dilke, K. (2005). Accelerated re-epithelialisation in β3-integrin-deficient mice is associated with enhanced TGFb-mediated signaling. Nat. Med. 11: 167-74.

McCarty, J.H., Cook, A. and Hynes, R.O. (2005). A novel interaction between αvβ8 integrin and band 4.1B via a highly conserved region of the band 4.1 C-terminal domain. PNAS 102:13479-13483.

Fang, J., Hodivala-Dilke, K., Johnson, B.D., Du, L.M., Hynes, R.O., White, G.C., and Wilcox, D.A. (2005). Therapeutic expression of the platelet-specific integrin, αIIbβ3, in a murine model for Glanzmann thrombasthenia. Blood. 106:2671-2679.

Lindenbach B.D., Evans, M.J., Syder, A.J., Wolk, B., Tellinghuisen, T.L., Liu, C.C., Maruyama, T., Hynes, R.O., Burton,, D.R., McKeating J.A. and Rice, C.M. (2005). Complete replication of Hepatitis C Virus in cell culture. Science 309; 623-626.

Wong, S.Y., Haack, H., Crowley, D., Barry, M., Bronson, R.T. and Hynes. R.O. (2005). Tumor-secreted VEGF-C is necessary for prostate cancer lymphangiogenesis, but lymphangiogenesis is unnecessary for lymph node metastasis. Cancer Res. 65: 9789-9798.

Taverna, D., Moher, H., Crowley, D., Borsig.L., Varki, A. and Hynes, R.O. (2004). Increased tumor growth in mice null for β3 or β3β5 integrins or selectins. Proc. Natl. Acad. Sci. USA. 101: 763-768.

Astrof, S., Crowley, D., George, E.L., Fukuda, T., Sekiguchi, K., Hanahan, D. and Hynes, R.O. (2004). Direct test of potential roles of EIIIA and EIIIB alternatively spliced segments of fibronectin in physiological and tumor angiogenesis. Mol. Cell. Biol. 24: 8662-8670.

Ni, H., Yuen, P.S.T., Papalia, J.M., Trevithick, J.E., Sakai, T., Fässler, R., Hynes, R.O. and Wagner, D.D. (2003) Plasma fibronectin promotes thrombus growth and stability in injured arterioles. Proc. Natl. Acad. Sci. USA 100: 2415-2419.

Hamano, Y., Zeisberg, M., Sugimoto, H., Lively, J.C., Maeshima, Y., Yang, C., Hynes, R.O., Werb, Z., Sudhakar, A. and Kalluri, R. (2003). Physiological levels of tumstatin, a fragment of collagen IV α3 chain, are generated by MMP-9 proteolysis and suppress angiogenesis via αVβ3 integrin. Cancer Cell 3:589-601.

Reynolds, L., Wyder, L., Lively, J.C., Taverna, D., Robinson, S.D., Huang, X., Sheppard, D, Hart, I., Hynes, R.O. and Hodivala-Dilke, K. (2002). Enhanced pathological angiogenesis in mice lacking β3-integrin or β3- and β5-integrins. Nature Medicine 8: 27-34.

Maeshima, Y., Sudhakar, A., Lively, J.C., Ueki, K., Kharbanda, S., Kahn, C.R., Sonenberg, N., Hynes, R.O. and Kalluri, R. (2002). Tumstatin, an endothelial cell-specific inhibitor of protein synthesis. Science 295: 140-143.

Borsig, L., Wong, R., Hynes, R.O., Varki, N.M. and Varki, A. (2002). Synergistic effect of L- and P-selectin in facilitating tumor metastasis in a syngeneic system involving non-mucin ligands – further evidence for selectin inhibition as a mode of heparin action. Proc. Natl. Acad. Sci. USA 99: 2193-8.

Francis, S.E., Goh, K.L., Hodivala-Dilke, K., Bader, B.L., Stark, M., Davidson, D. and Hynes, R.O. (2002). Central roles of α5β1 integrin and fibronectin in vascular development in mouse embryos and embryoid bodies. Arteriosclerosis, Thrombosis and Vasc. Biol. 22: 927-933.

McCarty, J.H., Monahan-Earley, R.A., Dvorak, A.M., Brown, L.F., Keller, M., Gerhardt, H., Rubin, K., Shani, M., Wolburg, H., Bader, B.L., Dvorak, H.F. and Hynes, R.O. (2002). Defective associations between blood vessels and developing neuronal parenchyma lead to cerebral hemorrhage in mice lacking av integrins. Mol. Cell Biol. 22: 7667-7677.

Whittaker, C.A. & Hynes, R.O. (2002). Distribution and evolution of von Willebrand/Integrin A domains: widely dispersed domains with roles in cell adhesion and elsewhere. Mol. Biol. Cell 13: 3369-3387.

Haack, H. and Hynes, R.O. (2001). Integrin receptors are required for cell survival and proliferation during development of the peripheral glial lineage. Devel. Biol. 233:38-55.

DiPersio, C.M., Trevithick, J.E. and Hynes, R.O. (2001) Functional comparison of the α3A/α3B cytoplasmic domain variants of the chicken α3 integrin subunit. Exp. Cell Res. 268: 45-60.

Rodriguez-Manzaneque, J.C., Lane, T.F., Ortega, M.A., Hynes, R.O., Lawler, J. and Iruela-Arispe, M.L. (2001). Thrombospondin-1 suppresses spontaneous tumor growth and angiogenesis and inhibits activation of matrix metalloprotease-9 and mobilisation of VEGF. Proc. Natl. Acad. Sci. USA 98: 12485-12490.

McHugh, K., Hodivala-Dilke, K., Zheng, M.H., Namba, N., Lam, J., Novack, D., Feng, X., Ross, F.P., Hynes, R.O. and Teitelbaum, S.L. (2000). Mice lacking avb3 integrins are osteosclerotic due to dysfunctional osteoclasts. J. Clin. Invest. 105: 433-440.

Rubin, G.M. et al. (2000). Comparative genomics of the eukaryotes. Science 287:2204-2215.

Hynes, R.O. and Zhao, Q. (2000). The evolution of cell adhesion. J.Cell Biol. 150: F89-F95.

Clark, E.A., Golub, T.R., Lander, E. and Hynes, R.O. (2000) Genomic analysis of metastasis reveals an essential role for rhoC. . Nature, 406: 532-535.

DiPersio, C.M., van der Neut, R., Georges-Labouesse, E., Kreidberg, J.A., Sonnenberg, A. and Hynes, R.O. (2000). α3β1 & α6β4 integrin receptors for laminin-5 are not essential for epidermal morphogenesis & homeostasis during skin development. J. Cell Sci. 113: 3051-62.

Ni, H., Denis, C.V., Subbarao, S., Degen, J.L., Sato, T.N., Hynes, R.O. and Wagner, D.D. (2000). Persistence of platelet thrombus formation in arterioles of mice lacking both von Willebrand factor and fibrinogen. J. Clin. Invest. 106: 385-392.

Arroyo, A.G., Taverna, D.T., Whittaker, C., Strauch, U., Bader, B.L., Rayburn, H., Crowley, D, Parker, C. and Hynes, R.O. (2000). In vivo roles of a4 integrins during leukocyte development and traffic: insights from the analysis of mice chimeric for α5, αv and α4 integrins. J. Immunology 165: 4667-75.

Hodivala-Dilke, K.M.,McHugh, K.,Tsakiris, D.A.,Rayburn, H.,Crowley, D.,Ullman-Culleré, M., Ross, F.P., Coller, B.S., Teitelbaum, S. & Hynes, R.O. (1999). β3-integrin-deficient mice: a model for Glanzmann thrombasthenia; placental defects & reduced survival. J.Clin.Invest. 103: 229-238.

Saoncella, S., Echtermeyer, F., Denhez, F., Nowlen, J.K., Mosher , D.F., Robinson, S.D., Hynes, R.O. and Goetinck, P.F. (1999). Syndecan-4 signals cooperatively with integrins, in a Rho-dependent manner, in the assembly of focal adhesions and actin stress fibers in fibronectin-null fibroblasts. Proc. Natl. Acad. Sci. USA 96: 2805-2810.

Bloom, L., Ingham, K.C. and Hynes, R.O. (1999) Fibronectin regulates assembly of actin filaments and focal contacts in cultured cells via the heparin-binding site in repeat III13. Mol. Biol. Cell 10:1521-1536.

Anderson, R., Fässler, R., Georges-Labouesse, E., Hynes, R.O., Bader, B., Kreidberg, J.A., Schaible, K., Heasman, J. and Wylie, C. (1999). Mouse primordial germ cells lacking β1 integrins enter the germ line but fail to migrate normally to the gonads. Development 126: 1655-1664.

Robinson, S.D., Frenette, P.S., Rayburn, H., Cummiskey, M., Ullman-Cullere, M., Wagner, D.D. and Hynes, R.O. (1999). Multiple targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc. Natl. Acad. Sci. USA 96: 11452-11457.

Calderwood, D.A., Zent, R., Rees, D.J.G., Hynes, R.O. and Ginsberg, M.H. (1999). The talin head domain binds to integrin b subunit cytoplasmic tails and regulates integrin activation. J.Biol. Chem. 274: 28071-28074.

Arroyo, A.G., Yang, J.T., Rayburn, H. and Hynes, R.O. (1999). α4 integrins regulate the proliferation differentiation balance of multilineage hematopoietic progenitors in vivo. Immunity 11: 555-566.

Fox, G.L., Rebay, I. and Hynes, R.O. (1999). Expression of Dfak56, a Drosophila homologue of vertebrate focal adhesion kinase, supports a role in cell migration in vivo. PNAS 96: 14978-14983.

Lawler, J., Sunday, M., Thibert, V., Duquette, M., George, E.L., Rayburn, H. and Hynes, R.O. (1998). Thrombospondin-1 is required for normal murine pulmonary homeostasis and its absence causes pneumonia. J. Clin. Invest. 101:982-992.

Crawford, S.E., Stellmach, V., Murphy-Ullrich, J., Ribeiro, S.F., Lawler, J., Hynes, R.O., Boivin, G.P., & Bouck, N. (1998). Thrombospondin is an activator of TGF-β in vivo. Cell 93:1158-70.

Dong, Z.M., Chapman, S.M., Brown, A.A., Frenette, P.S., Hynes, R.O. and Wagner, D.D. (1998). Combined role of P- and E-selectins in atherosclerosis. J. Clin. Invest. 102: 145-52.

Denis. C., Methia, N., Frenette, P.S., Rayburn, H., Ullman-Culleré, M., Hynes, R.O. and Wagner, D.D. (1998). A mouse model of severe von Willebrand disease: defects in hemostasis and thrombosis. Proc. Natl. Acad. Sci. USA. 95: 9524-9529.

Clark, E.A., King,W.G., Brugge, J.S., Symons, M. and Hynes, R.O. (1998) Integrin-mediated signals regulated by members of the Rho family of GTPases. J. Cell Biol. 142: 573-586.

Hodivala-Dilke, K.M, DiPersio, C. M., Kreidberg, J.A., and Hynes, R. O. (1998). Novel roles for α3β1 integrin as a regulator of cytoskeletal assembly and as a transdominant inhibitor of integrin receptor function in mouse keratinocytes. J. Cell Biol. 142: 1357-1369.

Borowsky, M.L. and Hynes, R.O. (1998). Layilin, a novel talin-binding transmembrane protein homologous with C-type lectins, is localized in membrane ruffles. J. Cell Biol. 143: 429-442.

Taverna, D., Disatnik, M.H., Rayburn, H., Bronson, R.T., Rando, T.A., Yang, J.T. and Hynes, R.O. (1998). Dystrophic muscle in mice chimeric for expression of a5 integrin. . J. Cell Biol. 143: 849-59.

Bader, B.L., Rayburn, H., Crowley, D. and Hynes, R.O. (1998) Extensive vasculogenesis, angiogenesis and organogenesis precede lethality in mice lacking all αv integrins. Cell 95: 507-519.

Hartwell, D.W., Butterfield, C.E., Frenette, P.S., Kenyon, B.M., Hynes, R.O., Folkman, J. and Wagner, D.D. (1998). Angiogenesis in P-selectin- and E-selectin-deficient mice. Microcirculation, 5: 173-8.

Radice, G., Rayburn, H., Matsunami, H., Knudsen, K. A., Takeichi, M. and Hynes, R.O. (1997) Developmental defects in mouse embryos lacking N-cadherin. Dev. Biol. 181: 64-78.

DiPersio, C. M., Hodivala-Dilke, K.M., Jaenisch, R. Kreidberg, J.A., and Hynes, R. O. (1997). α3β1 integrin is required for normal development of the epidermal basement membrane J. Cell Biol., 137: 729-742

Goh, K.L., Yang, J.T. and Hynes, R.O. (1997) Mesodermal defects and cranial neural crest apoptosis in a5 integrin-null embryos Development 124:4309-4319.

Stark, K.A., Yee, G.H., Roote, C.E., Williams, E.L., Zusman, S. and Hynes, R.O. (1997). A novel alpha integrin subunit associates with bPS and functions in tissue morphogenesis and movement during Drosophila development. Development 124:4583-4594.

Radice, G., Ferreira-Cornwell, M.C., Robinson, S.D., Rayburn, H., Chodosh, L.A., Takeichi, M. and Hynes, R.O. (1997). Precocious mammary gland development in P-cadherin-deficient mice. J.Cell Biol. 139: 1025-1032.

George, E.L., Baldwin, H.S. and Hynes, R.O. (1997). Fibronectins are essential for heart and blood vessel morphogenesis, but are dispensible for initial specification of precursor cells. Blood 90: 3073-86.

Frenette, P.S., Mayadas, T.N., Rayburn, H., Hynes, R.O. and Wagner, D.D. (1996) Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins. Cell 84: 563-574

Subramaniam, M., Frenette, P.S., Saffaripour, S., Johnson, R.C., Hynes, R.O. and Wagner, D.D. (1996). Defects in hemostasis in P-selectin-deficient mice Blood 87:1238-1242.

Tang, T., Frenette, P.S., Hynes, R.O., Wagner, D.D. and Mayadas, T.N. (1996). Cytokine-induced meningitis dramatically attenuated in mice deficient in endothelial selectins. J. Clin. Invest. 97:2485-90.

Arroyo, A. G., Yang, J. T., Rayburn, H. and Hynes, R. O. (1996) Differential requirements for α4 integrins during fetal and adult hematopoiesis. Cell 85: 997-1008.

Clark, E. A. and Hynes, R.O. (1996) Ras activation is necessary for integrin-mediated activation of extracellular signal-regulated kinase 2 and cytosolic phospholipase A2 but not for cytoskeletal organization. J. Biol. Chem. 271: 14814-14818.

Peters, J. H., and Hynes, R.O. (1996) Fibronectin isoform distribution in the mouse I. The alternatively spliced EIIIB, EIIIA and V segments show widespread codistribution in the developing mouse embryo. Cell Adhesion and Comm., 4: 103-125.

Peters, J. H., Chen, G., and Hynes, R.O. (1996) Fibronectin isoform distribution in the mouse II. Differential distribution of the alternatively spliced EIIIB, EIIIA and V segments in the adult mouse. Cell Adhesion and Comm., 4: 126-148.

Georges-Labouesse, E.N., George, E.L., Rayburn, H. and Hynes, R.O. (1996) Mesodermal development in mouse embryos mutant for fibronectin. Developmental Dynamics 207:145-56.

Yang, J. T., Rando, T., Rayburn, H.. Blau, H. and Hynes, R. (1996) Genetic analysis of α4β1 integrin functions in the development of mouse skeletal muscle. J.Cell Biol. 135:829-835.

Hemmings, L., Rees, D.J.G., Ohanian, V., Bolton, S.J., Gilmore, A.P., Patel, B., Priddle, H., Trevithick, J.E., Hynes, R.O. and Critchley, D.R. (1996) Talin contains three actin-binding sites each of which is adjacent to a vinculin-binding site. J. Cell Sci., 109: 2715-2726.

Yang, J.T., Rayburn, H. and Hynes , R. O. (1995). Cell adhesion events mediated by α4 integrins are essential in placental and cardiac development. Development 121:549-560.

DiPersio, C.M., Shah, S. and Hynes, R.O. (1995). α3Αβ1 integrin localizes to focal contacts in response to diverse extracellular ligands. J. Cell Sci. 108:2321-2336.

Grinblat, Y., Zusman, S., Yee, G., Hynes, R.O. and Kafatos, F.C. (1994). Functions of the cytoplasmic domain of the βPS integrin during Drosophila development. Development 120:91-102.

Spring, J., Paine-Saunders, S., Hynes, R.O. and Bernfield, M. (1994). Drosophila syndecan: a heparan sulfate proteoglycan with a cytoplasmic domain highly conserved in the vertebrate syndecan family. Proc. Natl. Acad. Sci. USA 91:3334-3338.

Gotwals, P.J., Fessler, L.I., Wehrli, M. and Hynes, R.O. (1994). Drosophila PS1 integrin is a laminin receptor and differs in ligand specificity from PS2. Proc. Natl. Acad. Sci. USA 91:11447-11451.

McLachlan, A.D., Stewart, M., Hynes, R.O. and Rees, D.J.G. (1994). Analysis of repeated motifs in the talin rod. J. Mol. Biol. 235:1278-1290.

Huh, G.S. and Hynes, R.O. (1994). Regulation of alternative pre-mRNA splicing by a novel repeated hexanucleotide element. Genes and Devel. 8:1561-1574.

Huh, G. and Hynes, R.O. (1993). Elements regulating an alternatively spliced exon of the rat fibronectin gene. Mol. Cell. Biol. 13:5301-5314.

Mayadas, T.N., Johnson R.C., Rayburn, H. , Hynes, R.O. and Wagner, D.D. (1993). Leukocyte rolling and extravasation severely compromised in P-selectin-deficient mice. Cell 74:541-54.

George, E.L., Georges-Labouesse, E.N., Patel-King, R.S., Rayburn, H. and Hynes, R.O. (1993). Defects in mesoderm, neural tube and vascular development in mouse embryos lacking fibronectin. Development 119:1079-1091.

Yang, J.T., Rayburn, H. and Hynes, R.O. (1993) Embryonic mesodermal defects in α5-integrin-deficient mice. Development 119:1093-1105.

Gilmore, A.P., Wood, C., Ohanian, V., Jackson, P., Patel, B., Rees, D.J.G., Hynes, R.O. and Critchley, D.R. (1993). The cytoskeletal protein talin contains at least two distinct vinculin binding domains. J. Cell Biol. 122:337-347.

Yee, G. H. and Hynes, R.O. (1993). A novel, tissue-specific integrin subunit, βν, expressed in the midgut of Drosophila melanogaster. Development 118:845-858.

Zusman, S., Grinblat, Y.,Yee, G., Kafatos, F.C. and Hynes, R.O. (1993). Functions of PS integrins during Drosophila development. Development 118:737-751.

DeSimone, D.W., Norton, P.A. and Hynes, R.O. (1992). Identification and characterization of alternatively spliced fibronectin mRNAs expressed in early Xenopus embryos. Dev. Biol. 149:357-9.

Guan, J.-L., Trevithick, J.E. and Hynes, R.O. (1991). Fibronectin/integrin interaction induces tyrosine phosphorylation of a 120 kDa protein. Cell Regulation 2:951-964.

Guan, J.-L. and Hynes, R.O. (1990). Lymphoid cells recognize an alternatively spliced segment of fibronectin via the integrin receptor α4β1. Cell 60:51-63.

Guan, J.-L., Trevithick, J.E. and Hynes, R.O. (1990). Retroviral expression of alternatively spliced forms of rat fibronectin. J. Cell Biol. 110:833-847.

Smith, J.C., Symes, K., Hynes, R.O. and DeSimone, D. (1990). Mesoderm induction and the control of gastrulation in Xenopus laevis: the roles of fibronectin and integrins. Development 108:229-238.

Zusman, S., Patel-King, R.S., ffrench-Constant, C. and Hynes, R.O. (1990). Requirements for integrins during Drosophila development. Development 108:391-402.

Marcantonio, E.E., Guan, J.-L., Trevithick, J.E. and Hynes, R.O. (1990). Mapping of the functional determinants of the integrin b1 cytoplasmic domain by site-directed mutagenesis. Cell Regulation 1:597-604.

Rees, D.J.G. Ades, S.A., Singer, S.J. and Hynes, R.O. (1990). Sequence and domain organization of talin, a member of a family of submembranous cytoskeletal proteins. Nature 347: 685-689.

Plantefaber, L.C. and Hynes, R.O. (1989). Changes in integrin receptors on oncogenically transformed cells. Cell 56:281-290.

Hynes, R.O., Marcantonio, E.E., Stepp, M.A., Urry, L.A. and Yee, G. (1989).Integrin heterodimer and fibronectin receptor complexity in avian and mammalian cells. J. Cell Biol. 109:409-420.

Solowska, J., Guan, J.-L., Marcantonio, E.E., Trevithick, J.E., Buck, C.A. and Hynes, R.O. (1989). Expression of normal and mutant avian integrin subunits in rodent cells. J. Cell Biol. 109:853-861.

ffrench-Constant, C. and Hynes, R.O. (1989). Alternative splicing of fibronectin is temporally and spatially regulated in the chicken embryo. Development 106:375-388.

ffrench-Constant, C., Van De Water, L., Dvorak, H.F. & Hynes, R.O. (1989). Reappearance of an embryonic pattern of fibronectin splicing during wound healing in the rat. J. Cell Biol. 109:903-14.

DeSimone, D.W. and Hynes, R.O. (1988). Xenopus integrins: structural conservation and evolutionary divergence of integrin b subunits. J. Biol. Chem. 263:5333-5340.

Marcantonio, E.E. and Hynes, R.O. (1988). Antibodies to the conserved cytoplasmic domain of the integrin b subunit react with proteins in vertebrates, invertebrates & fungi. J.Cell Biol. 106:1765-72.

ffrench-Constant, C. and Hynes R.O. (1988). Patterns of fibronectin gene expression and splicing during cell migration in chicken embryos. Development 104:369-382.

Lawler, J., Weinstein, R. and Hynes, R.O. (1988). Cell attachment to thrombospondin: the role of Arg-Gly-Asp, calcium and integrin receptors. J. Cell Biol. 107:2351-2361.

Schwarzbauer, J.E., Mulligan, R.C. and Hynes, R.O. (1987a). Efficient and stable expression of recombinant fibronectin polypeptides. Proc. Natl. Acad. Sci. USA. 84:754-758.

Patel, R.S., Odermatt, E., Schwarzbauer, J.E. and Hynes, R.O. (1987). Organization of the rat fibronectin gene provides evidence for "exon shuffling" during evolution. EMBO J. 6:2565-72.

Schwarzbauer, J.E., Patel, R.S., Fonda, D. and Hynes, R.O. (1987b). Multiple sites of alternative splicing of the rat fibronectin gene transcript. EMBO J. 6:2673-2580.

Hynes, R.O., Patel, R. and Miller, R.H. (1986). Migration of neuroblasts along preexisting axonal tracts during prenatal cerebellar development. J. Neuroscience 6:867-876.

Paul, J.I., Schwarzbauer, J.E., Tamkun, J.W. and Hynes, R.O. (1986). Cell-type-specific fibronectin subunits generated by alternative splicing. J. Biol. Chem. 261:12258-12265.

Tamkun, J.W., DeSimone, D.W., Fonda, D., Patel, R.S., Buck, C., Horwitz, A.F. and Hynes, R.O. (1986). Structure of integrin, a glycoprotein involved in the transmembrane linkage between fibronectin and actin. Cell, 46:271-282.

Lawler, J. and Hynes, R.O. (1986). The structure of human thrombospondin, an adhesive glycoprotein with multiple calcium-binding sites and homologies with several different proteins. J. Cell Biol. 103:1635-1648.

Cowin, P., Kapprell, H.-P., Franke, W.W., Tamkun, J.W. and Hynes, R.O. (1986). Plakoglobin: a plaque protein common to different kinds of intercellular adhering junctions. Cell 46:1063-73.

Price, J. and Hynes, R.O. (1985). Astrocytes in culture synthesize and secrete a variant form of fibronectin. J. Neuroscience, 15:2205-2211.

Odermatt, E., Tamkun, J.W. and Hynes, R.O. (1985). The repeating modular structure of the fibronectin gene: relationship to protein structure and subunit variation. Proc. Natl. Acad. Sci. USA. 82:6571-6575.

Gardner, J.M. and Hynes, R.O. (1985). Interaction of fibronectin with its receptor on platelets. Cell 42:439-448.

Tamkun, J.W., Schwarzbauer, J.E. and Hynes, R.O. (1984). A single rat fibronectin gene generates three different mRNAs by alternative splicing of a complex exon. Proc. Natl. Acad. Sci. USA 81:5140-5144.

Paul, J.I. and Hynes, R.O. (1984). Multiple fibronectin subunits and their posttranslational modifications. J. Biol. Chem. 21:13477-13487.

Lee, G., Hynes, R.O. and Kirschner, M. (1984). Temporal and spatial regulation of fibronectin in early Xenopus development. Cell 36:729-740.

Tamkun, J. and Hynes, R.O. (1983). Plasma fibronectin is synthesized and secreted by hepatocytes. J. Biol. Chem. 258:4641-4647.

Schwarzbauer, J.E., Tamkun, J.W., Lemischka, I.R. and Hynes, R.O. (1983). Three different fibronectin mRNAs arise by alternative splicing within the coding region. Cell 35:421-431.

Schwartz, M.A., Das, O.P. and Hynes, R.O. (1982). A new radioactive crosslinking reagent for studying the interactions of proteins. J. Biol. Chem. 257:2343-2349.

Wagner, D.D. and Hynes, R.O. (1982). Fibronectin-coated beads are endocytosed by cells and align with microfilament bundles. Exp. Cell Res. 140:373-381.

Bing, D.H., Almeda, S., Isliker, H., Lahav, J. and Hynes, R.O. (1982). Fibronectin binds to the Clq component of complement. Proc. Natl. Acad. Sci. USA 79:4198-4201.

Lahav, J., Schwartz, M.A. and Hynes, R.O. (1982). Analysis of platelet adhesion using a radioactive chemical crosslinking reagent: interaction of thrombospondin with fibronectin and collagen. Cell 31:253-262.

Van De Water, L., Schroeder, S., Crenshaw, E.B. and Hynes, R.O. (1981). Phagocytosis of gelatin-latex particles by a murine macrophage line is dependent on fibronectin & heparin. J. Cell Biol. 90:32-39.

Atherton, B.T. and Hynes, R.O. (1981). A difference between plasma and cellular fibronectins located with monoclonal antibodies. Cell 25:133-141.

Wagner, D.D., Ivatt, R., Destree, A.T. and Hynes, R.O. (1981). Similarities and differences between fibronectins of normal and transformed hamster cells. J. Biol. Chem. 256:11708-15.

Heasman, J., Hynes, R.O., Swan, A.P., Thomas, V. and Wylie, C.C. (1981). Primordial germ cells of Xenopus embryos: the role of fibronectin in their adhesion during migration. Cell 27:437-447.

Mayer B.W., Jr., Hay, E.D. and Hynes, R.O. (1981). Immunocytochemical localization of fibronectin in embryonic chick trunk and area vasculosa. Dev. Biol. 82:267-286.

Courtoy, P.J., Kanwar, Y.S., Hynes, R.O. and Farquhar, M.G. (1980). Fibronectin localization in the rat glomerulus. J. Cell Biol. 87:691-696.

Wagner, D.D. and Hynes, R.O. (1980). Topological arrangement of the major structural features of fibronectin. J. Biol. Chem. 255:4304-4312.

Senger, D.R., Wirth, D.F. and Hynes, R.O. (1980). Transformation-specific secreted phosphoproteins. Nature 286:619-621.

Senger, D.R., Wirth, D.F. and Hynes, R.O. (1979). Transformed mammalian cells secrete specific proteins and phosphoproteins. Cell 16:885-894.

Perkins, M.E., Ji, T.H. and Hynes, R.O. (1979). Crosslinking of fibronectin to proteoglycans at the cell surface. Cell 16:941-952.

Wagner, D.D. and Hynes, R.O. (1979). Domain structure of fibronectin and its relation to function. J. Biol. Chem. 254:6746-6754.

Choi, M. and Hynes, R.O. (1979). Biosynthesis and processing of fibronectin in NIL.8 hamster cells. J. Biol. Chem., 254:12050-12055.

Critchley, D.R., England, M.A., Wakely, J. and Hynes, R.O. (1979). Distribution of fibronectin in the ectoderm of gastrulating chick embryos. Nature 280:498-500.

Hynes, R.O. & Destree, A.T.(1978). 10 nm filaments in normal and transformed cells. Cell 13:151-63.

Ali, I.U. and Hynes, R.O. (1978). Effects of LETS glycoprotein on cell motility. Cell 14:439-446.

Schachner, M., Shoonmaker, G. and Hynes, R.O. (1978). Cellular and subcellular localization of LETS protein in the nervous system. Brain Res. 158:149-158.

Senger, D.R. and Hynes, R.O. (1978). C3 component of complement secreted by established cell lines. Cell 15:375-384.

Hynes, R.O. and Destree, A.T. (1978). Relationships between fibronectin (LETS protein) and actin. Cell 15:875-886.

Ali, I.U., Mautner, V.M., Lanza, R.P. and Hynes, R.O. (1977). Restoration of normal morphology, adhesion and cytoskeleton in transformed cells by addition of a transformation-sensitive surface protein. Cell 11:115-126.

Hynes, R.O. and Destree, A.T. (1977). Extensive disulfide bonding at the mammalian cell surface. Proc. Natl. Acad. Sci. 74:2855-2859.

Mautner, V.M. and Hynes, R.O. (1977). Surface distribution of LETS protein in relation to the cytoskeleton of normal and transformed fibroblasts. J. Cell Biol. 75:743-768.

Hynes, R.O., Martin, G.S., Shearer, M., Critchley, D.R. and Epstein, C.J. (1976). Viral transformation of rat myoblasts: effects on fusion and surface properties. Dev. Biol. 48:35-46.

Hynes, R.O. and Wyke, J.A. (1975). Alterations in surface proteins in chicken cells transformed by temperature-sensitive mutants of Rous sarcoma virus. Virology 64:492-504.

Smart, J.E. and Hynes, R.O. (1974). Developmentally regulated cell surface alterations in Dictyostelium discoideum. Nature 251:319-321.

Hynes, R.O. and Bye, J.M. (1974). Density and cell cycle dependence of cell surface proteins in hamster fibroblasts. Cell 3:113-120.

Hynes, R.O. (1973). Alteration of cell-surface proteins by viral transformation and by proteolysis. Proc. Natl. Acad. Sci. USA 70:3170-3174.

Hynes, R.O., Raff, R.A. and Gross, P.R. (1972). Properties of three cell types in sixteen-cell sea urchin embryos: aggregation and microtubule protein synthesis/RNA synthesis. Dev. Biol. 27:150-164 and 27:457-478.

Hynes, R.O. and Gross, P.R. (1970). A method for separating cells from early sea urchin embryos. Dev. Biol. 21:383-402.